博德特氏菌檢測(cè)試劑盒（PCR-熒光探針法）

廣州健侖生物科技有限公司

One tube multiplex for detection AND quantification of Bordela spp. and internal control.
單管多重檢測(cè)和定量博德特氏菌屬和內(nèi)控。

博德特氏菌檢測(cè)試劑盒（PCR-熒光探針法）

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

4、撈組織
當(dāng)組織載玻片置于40 oC溫水中之前，要先將水浴中的氣泡趕走，以免氣泡受熱上浮而貼到組織上，組織受熱展開，是組織不起皺紋，用載玻片撈組織時(shí)，一般取載玻片的下1/3或者下1/2一般每種組織撈5-6張，其中2-3張是備用的，每張載玻片上通常撈兩份組織，做對(duì)照使用，這樣形成的誤差就比較小了，而且撈載玻片的時(shí)候方向*，以便觀察，再將撈出來的載玻片置于架子上，放入37 oC溫箱中烘干。
5、脫蠟
依次將載玻片放入二甲苯-二甲苯-100%酒精-100%酒精-95%酒精-90%酒精-80%酒精-70%酒精，起脫蠟作用的主要是二甲苯，依據(jù)的是相似相溶的原理.一般在每個(gè)試劑中放10 min，天氣熱可以少放幾分鐘，相反，天氣較冷的話就要適當(dāng)延長(zhǎng)脫蠟時(shí)間，一般為12-15 min.
6、抗原修復(fù)
脫蠟后在清水中沖洗一段時(shí)間，加入3%H2O2浸泡10 min，從而除去內(nèi)源性的過氧化氫酶，然后倒掉H2O2，在清水中洗兩次，再加入檸檬酸緩沖液，放入微波爐中蒸煮3 min（中火），一般剛到沸騰即可，冷卻至室溫，然后再蒸煮一次，冷卻至室溫，蒸煮的目的是為了使抗原的位點(diǎn)暴露出來。
7、血清封閉
冷卻至室溫后，將檸檬酸緩沖液倒掉，水洗2次，并將載玻片置于PBS中5 min，洗2次，擦干組織周圍的PBS液，馬上加上血清，使一些非特異性的位點(diǎn)封閉起來，然后放入37 oC溫箱中半小時(shí)。血清稀釋10倍（900 ul PBS：100 ul血清封閉液）。

4, fishing organizations
When the tissue slides placed in 40 oC warm water, the first bubble in the water bath away, so as not to heat the bubbles floating and affixed to the tissue, heat tissue, it is best not to organize wrinkles, slide Tissue, the general take slides under 1/3 or 1/2 generally fishing 5-6 each tissue, of which 2-3 are spare, each slide is usually fishing two organizations, as a control Use, so the error is relatively small, but when fishing slides best in the same direction, in order to observe, and then remove the slide placed on the shelf, placed in a 37 oC incubator drying.
5, dewaxing
Slides were loaded sequentially into xylene-xylene-100% alcohol-100% alcohol-95% alcohol-90% alcohol-80% alcohol-70% alcohol, Similar to the principle of dissolving.Generally in each reagent put 10 min, the weather can be a few minutes less heat, on the contrary, the colder weather, it is necessary to extend the dewaxing time, usually 12-15 min.
6, antigen repair
After dewaxing, rinse in clean water for a period of time, add 3% H2O2 for 10 min to remove endogenous catalase, then drained H2O2, wash twice in clear water, add citric acid buffer, Microwave cooking 3 min (medium heat), usually just to boil, cooled to room temperature, and then digested again, cooled to room temperature, the purpose of cooking is to make the antigen site exposed.
7, serum closed
After cooling to room temperature, the citrate buffer was drained, washed twice with water, and the slide was placed in PBS for 5 min, washed 2 times, the PBS solution around the tissue was dried, and immediay added serum to make some non-specific The site was closed and then placed in a 37 oC incubator for half an hour. Serum was diluted 10-fold (900 ul PBS: 100 ul serum blocking solution).