- 產(chǎn)品描述
PCR試劑 流感病毒AB/合胞病毒AB四聯(lián)檢測試劑盒
廣州健侖生物科技有限公司
One tube multiplex for detection of influenza A, B, respiratory syncytial virus A/B and internal control.
一管多重檢測流感A,B,呼吸道合胞病毒A / B和內(nèi)部控制。
Principle
Multiplex real-time PCR for detection of pathogen genes by TaqMan® technology
Targets
One tube multiplex PCR:
influenza A virus
influenza B virus
human respiratory syncytial viruses A/B
internal Control
Specimen
This test is for use with extracted nucleic acid from respiratory samples (throat/nasal swabs, bronchoalveolar lavage and sputum) of human origin
Storage
-20°C
Shelf life
12 months from manufacture
PCR試劑 流感病毒AB/合胞病毒AB四聯(lián)檢測試劑盒
貨號 | 產(chǎn)品名稱 | 英文名稱 |
JL-FT001 | 呼吸道病原體21種多重檢試劑盒(PCR方法) | Respiratory pathogens 21 |
JL-FT002 | 21種呼吸道病原體聯(lián)合檢試劑盒(PCR方法) | Respiratory pathogens 21 |
JL-FT003 | 呼吸道病原體25聯(lián)檢測試劑盒(PCR方法) | Respiratory pathogens 25 plus |
JL-FT004 | 33種呼吸道病原體聯(lián)合檢測試劑盒(PCR方法) | Respiratory pathogens 33 |
JL-FT005 | 8種細菌性肺炎多重檢測試劑盒(PCR方法) | Bacterial pneumoniae CAP |
JL-FT006 | 4種非典型肺炎聯(lián)合檢測試劑盒(PCR方法) | Atypical CAP |
JL-FT007 | 肺炎克雷伯菌/銅綠假單胞菌聯(lián)合檢測試劑盒(PCR方法) | Bacterial pneumoniae HAP |
JL-FT008 | 博德特氏菌檢測試劑盒(PCR-熒光探針法) | Bordela |
JL-FT009 | 3種流感病毒檢測試劑盒(PCR-熒光探針法) | FLU |
JL-FT010 | 中東呼吸綜合征冠狀病毒(MERS-CoV)檢測試劑盒(PCR方法) | MERS-CoV |
JL-FT011 | MERS-CoV 中東呼吸綜合征冠狀病毒PCR檢測試劑盒 | MERS-CoV |
JL-FT012 | 卡氏肺孢子蟲檢測試劑盒(PCR-熒光探針法) | Pneumocystis jirovecii |
JL-FT013 | 流感甲乙型/人呼吸道合胞病毒AB型四聯(lián)檢測試劑盒(PCR-熒光探針法) | FLU/HRSV |
JL-FT014 | 人呼吸道合胞病毒AB型和流感病毒甲乙型聯(lián)合檢測PCR試劑盒 | FLU/HRSV |
JL-FT015 | 軍團菌屬三通道多重檢測試劑盒(PCR-熒光探針法) | Legionella |
JL-FT016 | 人冠狀病毒NL63、 229E、OC43 and HKU1聯(lián)合檢測試劑盒(PCR方法) | HCoV |
我司還提供其它進口或國產(chǎn)試劑盒:登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。
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PCR試劑
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【公司名稱】 廣州健侖生物科技有限公司
【市場部】 楊永漢
【】
【騰訊 】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室
9.封閉:在山羊血清封閉,非特異性染色仍然較強時,可延長封閉時間或用濃縮血清封閉
10.抗體稀釋:應遵循“現(xiàn)用現(xiàn)配”的原則,對于PBS稀釋的抗體一定要當天使用。
11.背景高:在抗體濃度、反應時間、反應溫度等合適的條件下,如果背景依舊高,可采用含1‰ Tween20的PBS洗,特別是在顯色之前要多洗。
12.返藍:在蘇木素復染后,可用堿性緩沖液(如PBS)或Na2HPO4的飽和溶液返藍。
13.顯色:一定要在顯微鏡下觀察,注意控制背景。
DAB法 顯示過氧化物酶
〔原理〕 過氧化物酶分解H2O2的過程中,下面反應不直接發(fā)生:AH2(供氫體)+H2O2→A(供氫體的氧化物)+2H2O2實驗可知,有稱為復合物 Ⅰ、Ⅱ、Ⅲ、的酶——底物(受氫體)復合體生成,在復合體zui后分解為酶和水時,游離的原子氧使供氫體氧化而顯色。酶組織化學中所使用的供氫體應是含有色原性基團的發(fā)色物質(zhì),如奈酚和聯(lián)苯胺。
免疫組化染色步驟(以ABC法為例)
9. Blocking: When goat serum is closed and nonspecific staining is still strong, it can be extended or closed with concentrated serum
10. Antibody dilution: Follow the principle of "Ready-to-use Ready-to-use". Antibody diluted with PBS must be used on the same day.
11. Background High: If the background is still high, under appropriate conditions of antibody concentration, reaction time, reaction temperature, etc., wash with PBS containing 1 ‰ Tween20, especially before developing.
12. Back to blue: In the hematoxylin counterstain, available alkaline buffer (such as PBS) or Na2HPO4 saturated solution back to blue.
13. Color: Be sure to observe under the microscope, pay attention to control the background.
DAB method shows peroxidase
[Principle] In the process of decomposition of H2O2 by peroxidase, the following reaction does not directly occur: AH2 (hydrogen donor) + H2O2 → A (hydrogen donor oxide) + 2H2O2 experiment shows that there are complex Ⅰ, Ⅱ , Ⅲ, the enzyme (substrate) (substrate) is generated by the complex, the final decomposition of the complex enzyme and water, the free atomic oxygen oxidation of hydrogen donor and color. Hydrogen donors used in enzymatic histochemistry should be chromogenic substances containing chromogenic groups, such as naloxol and benzidine.
Immunohistochemical staining steps (ABC method as an example)