細(xì)菌性腦膜炎3聯(lián)熒光PCR檢測(cè)試劑盒

廣州健侖生物科技有限公司

單管多重檢測(cè)和定量肺炎鏈球菌，腦膜炎奈瑟氏菌，流感嗜血桿菌和內(nèi)部控制。
One tube multiplex for detection AND quantification of Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae and internal control.

細(xì)菌性腦膜炎3聯(lián)熒光PCR檢測(cè)試劑盒

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

3．濾紙保藏法
（1）將濾紙剪成0．5×1．2cm的小條，裝入0．6×8cm的安瓿管中，每管1—2張，塞以棉塞，1．05kg/cm2>，121．3℃滅菌30分鐘。
（2）將需要保存的菌種，在適宜的斜面培養(yǎng)基上培養(yǎng)，使充分生長。
（3）取滅菌脫脂牛乳1—2ml滴加在滅菌培養(yǎng)皿或試管內(nèi)，取數(shù)環(huán)菌苔在牛乳內(nèi)混勻，制成濃懸液。
（4）用滅菌鑷子自安瓿管取濾紙條浸入菌懸液內(nèi)，使其吸飽，再放回至安瓿管中，塞上棉塞。
（5）將安瓿管放入內(nèi)有五氧化二磷作吸水劑的干燥器中，用真空泵抽氣至干。
（6）將棉花塞入管內(nèi)，用火焰按圖Ⅶ-13熔封，保存于低溫下。
（7）需要使用菌種，復(fù)活培養(yǎng)時(shí)，可將安瓿管口在火焰上燒熱，滴一滴冷水在燒熱的部位，使玻璃破裂，再用鑷子敲掉口端的玻璃，待安瓿管開啟后，取出濾紙，放入液體培養(yǎng)基內(nèi)，置溫箱中培養(yǎng)。

This method is practical and effective. Molds, actinomycetes, spores and bacteria can be preserved more than 2 years immortality, yeast can be stored for 1-2 years, the general non-spore bacteria can be stored for about 1 year, even with the usual method is difficult to preserve meningococci, at 37 ℃ Thermostat, can also be stored for 3 months. This method has the advantage of making a simple, no special equipment, and do not need to move frequently. The disadvantage is that the preservation must be placed upright, occupy a larger location, but also inconvenient to carry. Care should be taken when the culture rings are burned in the flame after the paraffin is removed from the liquid paraffin and the culture is easily splashed together with residual liquid paraffin.
3. Filter paper preservation method
(1) The filter paper cut into 0.5 × 1.2cm strip into the 0.6 × 8cm ampoule tube, each tube 1-2, tampon plug, 1.05kg / cm2>, 121 Sterilize at 3 ° C for 30 minutes.
(2) The need to save the strains, cultured in the appropriate slant medium, so that full growth.
(3) Take 1-2ml sterile skim milk drop in sterile petri dishes or test tube, take a few ring bacteria in the milk mix, made into a suspension.
(4) using sterile tweezers to take the filter paper from ampoule tube immersed in the bacterial suspension, make it saturated, and then back into the ampoule tube, tampon plug.
(5) Place the ampoule into a desiccator containing phosphorus pentoxide as a water-absorbing agent and pump it to dryness with a vacuum pump.
(6) The cotton into the tube, flame with Figure VII-13 seal, stored at low temperature.
(7) need to use bacteria, the resurrection culture, the ampoule nozzle can be heated in the flame, a drop of cold water in the hot parts, so that the glass rupture, and then tweezers knock the mouth of the glass until the ampoule tube open , Remove the filter paper, into the liquid medium, incubator incubator.
Bacteria, yeasts, filamentous fungi can be preserved by this method, the first two can be stored for about 2 years, some filamentous fungi can even be preserved for 14-17 years. This method is more liquid nitrogen, freeze-drying method is simple, does not require special equipment.