神經(jīng)9項(xiàng)聯(lián)合檢測試劑盒（PCR-熒光探針法）

廣州健侖生物科技有限公司

Four tube multiplex for detection of cytomegalovirus, Epstein-Barr virus, adenovirus, herpes simplex virus 1, 2, varicella-zoster virus, enterovirus, parechovirus, human herpes virus 6, 7, parvovirus B19 and internal control.
四管多重檢測巨細(xì)胞病毒，EB病毒，腺病毒，單純皰疹病毒1,2型，水痘帶狀皰疹病毒，腸道病毒，小RNA病毒，人皰疹病毒6,7型，細(xì)小病毒B19和內(nèi)部對照。

神經(jīng)9項(xiàng)聯(lián)合檢測試劑盒（PCR-熒光探針法）

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

（1）取河沙加入10％稀鹽酸，加熱煮沸30分鐘，以去除其中的有機(jī)質(zhì)。
（2）倒去酸水，用自來水沖洗至中性。
（3）烘干，用40目篩子過篩，以去掉粗顆粒，備用。
（4）另取非耕作層的不含腐植質(zhì)的瘦黃土或紅土，加自來水浸泡洗滌數(shù)次，直至中性。
（5）烘干，碾碎，通過100目篩子過篩，以去除粗顆粒。
（6）按一份黃土、三份沙的比例（或根據(jù)需要而用其他比例，甚至可全部用沙或全部用土）摻合均勻，裝入10×100mm的小試管或安瓿管中，每管裝1g左右，塞上棉塞，進(jìn)行滅菌，烘干。
（7）抽樣進(jìn)行無菌檢查，每10支沙土管抽一支，將沙土倒入肉湯培養(yǎng)基中，37℃培養(yǎng)48小時，若仍有雜菌，則需全部重新滅菌，再作無菌試驗(yàn)，直至證明無菌，方可備用。
（8）選擇培養(yǎng)成熟的（一般指孢子層生長豐滿的，營養(yǎng)細(xì)胞用此法效果不好）優(yōu)良菌種，以無菌水洗下，制成孢子懸液。
（9）于每支沙土管中加入約0．5ml（一般以剛剛使沙土潤濕為宜）孢子懸液，以接種針拌勻。
（10）放入真空干燥器內(nèi)，用真空泵抽干水分，抽干時間越短越好，務(wù)使在12小時內(nèi)抽干。
（11）每10支抽取一支，用接種環(huán)取出少數(shù)沙粒，接種于斜面培養(yǎng)基上，進(jìn)行培養(yǎng)，觀察生長情況和有無雜菌生長，如出現(xiàn)雜菌或菌落數(shù)很少或根本不長，則說明制作的沙土管有問題，尚須進(jìn)一步抽樣檢查。
（12）若經(jīng)檢查沒有問題，用火焰熔封管口，放冰箱或室內(nèi)干燥處保存。每半年檢查一次活力和雜菌情況。
（13）需要使用菌種，復(fù)活培養(yǎng)時，取沙土少許移入液體培養(yǎng)基內(nèi)，置溫箱中培養(yǎng)。
4. Sandy soil preservation method
(1) Take sand and river add 10% dilute hydrochloric acid, heat and boil for 30 minutes to remove the organic matter therein.
(2) pour acid, rinse with tap water until neutral.
(3) drying, with 40 mesh sieve to remove coarse particles, spare.
(4) Take another non-tillage layer of humus-free thin loess or laterite, add tap water to wash several times until neutral.
(5) drying, crushing, sieved through a 100 mesh sieve to remove coarse particles.
(6) Mix well in a ratio of one loess and three sands (or in all other proportions, or even all with sand or soil, if needed) and load into small test tubes or ampoules of 10 x 100 mm each Install about 1g, stuffed tampons, sterilization, drying.
(7) Sampling for aseptic inspection, pumping one out of 10 sand soil tubes, pouring sand into broth medium, incubating at 37 ℃ for 48 hours, if there is still bacteria, you need to re-sterilize, then no Bacteria test, until proven sterile, before use.
(8) choose to c*te mature (generally refers to the spore layer full growth, vegetative cells with this method ineffective) good strains, sterile water washing, made of spore suspension.
(9) Add about 0.5ml (generally only to moistening the soil) spore suspension to each sand tube and mix it with an inoculation needle.
(10) into a vacuum desiccator, pumping the water with a vacuum pump, the pumping time is as short as possible, so that within 12 hours drained.
(11) Extract one for each 10, remove a few grit with inoculation loop, inoculate on slant culture medium, culture, observe the growth and presence or absence of bacteria growth, such as the emergence of a small number of bacteria or colonies or not at all Long, then the production of sand tube problems, still need further sampling.
(12) If there is no problem after inspection, flame-seal the nozzle, put the refrigerator or indoor dry place to save. Check the vitality and bacteria every six months.
(13) need to use bacteria, resurrection culture, take a little sand into the liquid medium, incubator incubator.
This method is mostly used to produce spores of microorganisms such as mold, actinomycetes, and therefore the most widely used antibiotic industrial production, the effect is also good, can be stored for about 2 years, but applied to vegetative cells ineffective.